Ir a pucv.cl

Labor académica

TESIS DE DOCTORADO

Tesistas Titulo Programa Año
Nadia Guajardo Evaluación de la selectividad de lipasas inmovilizadas en reacciones de síntesis asimétricas. Profesora Guía: Lorena Wilson; Profesora Co-Guía: Zaida Cabrera. Doctorado en Ciencias de la Ingeniería con mención en Ingeniería Bioquímica 2014

PROYECTOS

Proyectos con financiamiento Externo

Asymmetric synthesis of chiral compounds at high concentrations of substrate catalyzed by immobilized and stabilized enzymes. Investigador Principal. FONDECYT 1130981 2013-2015
Consolidación de Naturalis como puente para la aplicación del conocimiento científico desde Chile para el mundo. Subproyecto Nº5 del Proyecto “Fosfolipidos estructurados para la prevención y tratamiento de dislipidemias y cáncer” Asesor Técnico. CORFO INNOVA 12CTI-16838 2013-2015
Desarrollo de un agente biológico para su aplicación industrial en el proceso de blanqueamiento de la pulpa de celulosa. Ingeniero Investigador INNOVA 10IEI-7152 2010-2012
Reacciones asimétricas catalizadas por enzimas inmovilizadas y estabilizadas. Investigador Responsable. FONDECYT 11090321 2009-2012

PUBLICACIONES

Publicaciones en revistas

  1. Guajardo, N.; Bernal, C.; Wilson, L.; Cabrera, Z., Asymmetric hydrolysis of dimethyl-3-phenylglutarate in sequential batch reactor operation catalyzed by immobilized Geobacillus thermocatenulatus lipase.; Catalysis Today, En prensa DOI: 10.1016/j.cattod.2014.12.039., 2015
  2. Palomo, J.M., Cabrera, Z., Enzymatic Desymmetrization of Prochiral Molecules.; Current Organic Synthesis, 9, 791-805, 2012
  3. Cabrera, Z.; Palomo, J.M., Enantioselective Desymmetrization of prochiral diesters catalyzed by immobilized Rhizopus oryzae lipase.; Tetrahedron: Asymmetry, 22, 2080-2084, 2011
  4. Cabrera, Z., Palomo, J.M. 

    Enantioselective Desymmetrization of prochiral diesters catalyzed by immobilized Rhizopus oryzae lipase. ; Tetrahedron: Asymmetry, doi:10.1016/j.tetasy 2011.11.012 , 2011
  5. Cabrera, Z., Gutarra, M., Guisan, J.M., Palomo, J.M. , Highly enantioselective biocatalysts by coating immobilized lipases with Polyethyleneimine. ; Catalysis Communications, , 11, 964–967. , 2010
  6. Cabrera, Z.; Fernandez-Lorente, G.; Fernandez-Lafuente, R.; Palomo, J. M.; Guisán, J. M. 

    Novozym 435 displays very different selectivity compared to lipase from Candida antarctica B adsorbed on other hydrophobic supports; Journal of Molecular Catalysis B: Enzimatic,, 57, 171-176. , 2009
  7. Fernández-Lorente, G., Cabrera, Z., Godoy, C., Fernández-Lafuente, R., Palomo, J. M., Guisán, J.M. , Interfacially activated Lipases against hydrophobic supports: effect of the support nature on the biocatalytic properties.; Process Biochemistry, , 43; 1061-1067, 2008
  8. Cabrera, Z., Fernández-Lorente, G., Fernández-Lafuente, R., Palomo, J. M., Guisán, J.M.,Asymmetric hydrolysis of dimethyl 3-phenylglutarate catalyzed by Lecitase Ultra® Effect of the immobilization protocol on its catalytic properties; Enzyme and Microbial Technology, 43; 531-536. , 2008
  9. Cabrera, Z., Fernández-Lorente, G., Fernández-Lafuente, R., Palomo, J. M., Guisán, J.M.,Enhancement of Novozyme-435 catalytic properties by physical or chemical modification.; Process Biochemistry, , 44, 226-231. , 2008
  10. Cabrera, Z., Palomo, J.M., Fernández-Lorente, G., Guisán, J. M., Fernández-Lafuente, R. ,Partial and Enantioselective Hydrolysis of diethyl phenylmalonate by immobilized preparations of lipase from Thermomyces lanuginosa.; Enzyme and Microbial technology, 40, 1280-1285. , 2007
  11. Cabrera, Z., Lopez-Gallego, F., Fernández-Lorente, G., Palomo, J.M., Montes, T., Grazu, V., Guisán, J. M., Fernández-Lafuente, R. , Asymmetric hydrolysis of dimethyl phenylmalonate by immobilized preparations of Penicillin G. acylase from E. coli.; Enzyme and Microbial Technology,, 40, 997-1000. , 2007
  12. Fernández-Lorente, G., Palomo, J.M., Cabrera, Z., Guisán, J.M., Fernandez-Lafuente, R. ,Specificity enhancement towards hydrophobic substrates by immobilization of lipases by interfacial activation on hydrophobic supports.; Enzyme and Microbial Technology, , 41, 565-569., 2007
  13. Illanes, A., Cabrera, Z., Wilson, L., Aguirre, C., Synthesis of cephalexin in ethylene glycol with glyoxyl-agarose immobilised penicillin Acylase: temperature and pH optimisation; Process Biochemistry, 39,111-117, 2003
  14. Rocha, J., Vega, D., Cabrera, Z., Bolivar, J. M., Fernandez-Lafuente, R., Berenguer, J., Guisán, J.M. , Purification, immobilization and stabilization of a highly enantioselective alcohol dehydrogenase from Thermus thermophilus HB27 cloned in E. coli.; Process Biochemistry,, 44, 1004-1012, 2009
  15. Fernández-Lorente, G., Palomo, J.M., Cabrera, Z., Fernández-Lafuente, R, Guisán, J.M. ,Improved catalytic properties of immobilized lipases by the presence of very low concentrations of detergents in the reaction medium.; Biotechnology Bioengineering, , 97, 242-250., 2007
  16. Illanes, A., Wilson, L., Altamirano, C., Cabrera, Z., Alvarez, L., Aguirre, C. , Production of cephalexin in organic medium at high substrate concentrations with CLEA of penicillin acylase and PGA-450.; Enzyme and Microbial Technology, , 40 195–203., 2007
  17. Fernández-Lorente, G., Palomo, J. M., Mateo, C., Munilla, R., Ortiz, C., Cabrera, Z., Guisán, J.M., Fernández-Lafuente, R., Glutaraldehyde crosslinking of lipases adsorbed on aminated supports in the presence of detergents leads to improved performance.; Biomacromolecules, 7, 2610-2615., 2006